Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) Is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2. Both genes are nuclear, located on chromosome 4 and 5 respectively (in Arabidopsis). The protein products, PEPCK1 and PEPCK2, are both highly conserved in the model plant, Arabidopsis thaliana.

Price 126 € AS08 300 | extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection. PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. PEB plant & algal protein extraction bufferThe use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the quantity of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference). The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure) depending on the starting material.